FRT-seq: Amplification-free, strand-specific, transcriptome sequencing

FRT-seq: Amplification-free, strand-specific, transcriptome sequencing by Lira Mamanova et. al. published in Nat Methods. 2010 February; 7(2): 130–132.

Abstract

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided. Since the template is poly A+ RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-ended sequencing.