High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis by Rupali P Patwardhan et. al. published in Nat Biotechnol. 2009 December; 27(12): 1173–1175.

We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three bacteriophage promoters and three mammalian core promoters in a single experiment per promoter. The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology.