ChIP-seq and beyond: new and improved methodologies to detect and characterize protein-DNA interactions.




ChIP-seq and beyond: new and improved methodologies to detect and characterize protein-DNA interactions by Furey, Terrence S published in Nature reviews. Genetics. 2012 13(12):840-52 Abstract Chromatin immunoprecipitation experiments followed by sequencing (ChIP-seq) detect protein-DNA binding events and chemical modifications of histone proteins. Challenges in the standard ChIP-seq protocol have motivated recent enhancements in this approach, such as […]






Genome-wide maps of chromatin state in pluripotent and lineage-committed cells.




Genome-wide maps of chromatin state in pluripotent and lineage-committed cells., by Tarjei S. Mikkelsen et. al. published in Nature. 2007 August 2; 448(7153): 553–560. Abstract We report the application of single molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over 4 billion bases of sequence from chromatin immunoprecipitated DNA, […]