Global gene disruption in human cells to assign genes to phenotypes




Global gene disruption in human cells to assign genes to phenotypes by Jan E. Carette, et. al. published in Nat Biotechnol. 2011 May 29; 29(6): 542–546. Abstract Insertional mutagenesis in a haploid background can lead to complete disruption of gene function1. Here we generate a population of human cells that contain insertions in >98% of […]