FRT-seq: Amplification-free, strand-specific, transcriptome sequencing




FRT-seq: Amplification-free, strand-specific, transcriptome sequencing by Lira Mamanova et. al. published in Nat Methods. 2010 February; 7(2): 130–132. Abstract We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and […]