Mapping and quantifying mammalian transcriptomes by RNA-Seq




Mapping and quantifying mammalian transcriptomes by RNA-Seq by Mortazavi, A. et. al. published in Nat Methods. 2008 Jul;5(7):621-8. Epub 2008 May 30. Abstract We have mapped and quantified mouse transcriptomes by deeply sequencing them and recording how frequently each gene is represented in the sequence sample (RNA-Seq). This provides a digital measure of the presence […]






Transcriptome Assembly and Isoform Expression Level Estimation from Biased RNA-Seq Reads




Transcriptome Assembly and Isoform Expression Level Estimation from Biased RNA-Seq Reads, by Wei Li and Tao Jiang from University of California, Riverside, CA, USA and Tsinghua University, Beijing, China. Abstract Motivation: RNA-Seq uses the high-throughput sequencing technology to identify and quantify transcriptome at an unprecedented high resolution and low cost. However, RNA-Seq reads are usually […]