High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis




High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis by Rupali P Patwardhan et. al. published in Nat Biotechnol. 2009 December; 27(12): 1173–1175. We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed […]